Protein Purification Reagents and Kits
Enzo Life Sciences Granzyme B assay kit for drug discovery (1x96wells)
Granzyme B is a serine protease found in the lymphocyte granules of cytotoxic T-lymphocytes (CTLs) and natural killer (NK) cells. Activated granzyme B prefers an aspartic acid residue at the P1 site of its substrates; a preference shared by only one other group of mammalian proteases, the caspases. Granzyme B is required for the rapid apoptotic signal delivered by CTLs and NK cells, an effect mediated by its ability to cleave and activate various procaspases. Granzyme B plays a crucial role in the development of acute graft-vs.-host disease. Thus, the inhibition of this enzyme may prove to be an important tool in controlling immune responses. Conversely, the failure of granzyme B-induced apoptosis may contribute to certain pathologies. Applications: Colorimetric detection, Fluorescent detection, HTS Activity assay.
STEMCELL Technologies RoboSep™ Human B Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Human B Cell Isolation Kit is designed to isolate B cells from fresh or previously frozen peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched, desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. This product can be used in place of the EasySep™ Human B Cell Enrichment Kit (Catalog #19054) for even faster cell isolations.
STEMCELL Technologies EasySep™ Rat IgM Depletion Kit, For processing: 1 x 10^9 cells
The EasySep™ Rat IgM Depletion Kit is designed to deplete IgM+ cells from single-cell suspensions of splenocytes or lymph nodes or from whole blood by positive selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.
STEMCELL Technologies RosetteSep™ CTC Enrichment Cocktail Containing Anti-CD36, For processing: 200 mL whole blood processed
The RosetteSep™ CTC Enrichment Cocktail Containing Anti-CD36 is designed to enrich circulating epithelial tumor cells from fresh whole blood by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD2, CD16, CD19, CD36, CD38, CD45, CD66b and glycophorin A on red blood cells (RBCs). When centrifuged over a density gradient medium such as Lymphoprep™ (Catalog #07801), the unwanted cells pellet along with the RBCs. The purified tumor cells are present as a highly enriched population at the interface between the plasma and the density gradient medium.This cocktail is compatible with small-cell carcinoma samples, including lung cancer samples. If you are working with breast cancer samples, use the RosetteSep™ CTC Enrichment Cocktail Containing Anti-CD56 (Catalog #15137).
STEMCELL Technologies RoboSep™ Human CD8+ T Cell Enrichment Kit with Filter Tips, For processing: 1 x 10^9 cells
The EasySep™ Human CD8+ T Cell Enrichment Kit is designed to isolate CD8+ T cells from fresh or previously frozen peripheral blood mononuclear cells or ammonium chloride-lysed leukapheresis sample by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-CD8+ T cells and glycophorin A and dextran-coated magnetic particles. The labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.For even faster cell isolations, we recommend the new EasySep™ Human CD8+ T Cell Isolation Kit (17953) (http://www.stemcell.com/en/Products/All-Products/EasySep-Human-CD8-T-Cell-Isolation-Kit.aspx) which isolates cells in just 8 minutes.
STEMCELL Technologies RoboSep™ HLA B Cell Enrichment: Complete Processing Kit for Whole Blood with Filter Tips, For processing: 200 mL whole blood processed
The EasySep™ HLA B Cell Enrichment Kit: Complete Processing Kit for Whole Blood is designed to isolate human B cells from HetaSep™-treated whole blood by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD2, CD3, CD14, CD16, CD33, CD36, CD41, CD43, CD56, CD66b, glycophorin A and dextran-coated magnetic particles. The labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.
STEMCELL Technologies EasySep™ Human PE Positive Selection Kit II, For processing: 1 x 10^9 cells
The EasySep™ Human PE Positive Selection Kit II is designed to isolate cells that are labeled with PE (phycoerythrin)-conjugated antibodies by positive selection. Desired cells are targeted with antibody complexes recognizing PE and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.This product replaces the EasySep™ Human PE Positive Selection Kit (Catalog #18551). For highly purified particle-free cells, we recommend the EasySep™ Release Human PE Positive Selection Kit (Catalog #17654) (https://www.stemcell.com/products/easysep-release-human-pe-positive-selection-kit.html)
STEMCELL Technologies RosetteSep™ HLA Granulocyte Depletion Cocktail, For processing: 1000 mL whole blood processed
Immunodensity depletion of granulocytes for HLA analysis
STEMCELL Technologies RoboSep™ APC Positive Selection Kit II, For processing: 1 x 10^9 cells
The EasySep™ APC Positive Selection Kit II is designed to isolate cells that are labeled with APC (allophycocyanin)-conjugated antibodies by positive selection. Desired cells are targeted with antibody complexes recognizing APC and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.This product replaces the EasySep™ APC Positive Selection Kit (Catalog #18453) for even faster cell isolations.
STEMCELL Technologies EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit, For processing: 1 x 10^9 cells
This kit isolates human CD4+CD127lowCD25+ regulatory T cells (Tregs) from fresh or previously frozen peripheral blood mononuclear cells (PBMCs) or leukapheresis samples. First, CD25+ cells are isolated by column-free immunomagnetic positive selection using EasySep™ Releasable RapidSpheres™. Then, bound magnetic particles are removed from the EasySep™-isolated CD25+ cells, and unwanted non-Tregs are targeted for depletion. The final isolated fraction contains highly purified CD4+CD127lowCD25+ cells that express high levels of FOXP3 and are immediately ready for downstream applications. An optional protocol allows for the isolation of CD4+CD25- responder T cells in parallel for use in functional studies. Following cell isolation with this EasySep™ kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins, or other chemically related ligands.
STEMCELL Technologies EasySep™ Non-Human Primate T Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Non-Human Primate T Cell Isolation Kit is designed to isolate T cells from fresh peripheral blood mononuclear cells by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.This kit has been verified for use with rhesus and cynomolgus macaques.
STEMCELL Technologies RosetteSep™ HLA Total Lymphocyte Enrichment Cocktail, For processing: 1000 mL whole blood processed
Immunodensity isolation of untouched lymphocytes for HLA analysis
STEMCELL Technologies RoboSep™ Human CD4+ T Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Human CD4+ T Cell Isolation Kit is designed to isolate CD4+ T cells from fresh or previously frozen peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.This product can be used in place of the EasySep™ Human CD4+ T Cell Enrichment Kit (Catalog #19052) for even faster cell isolations.
STEMCELL Technologies RosetteSep™ HLA Lymphoid Cell Enrichment Kit, Size: 20 Tests
Immunodensity isolation of untouched CD3+ lymphocytes for lineage-specific chimerism analysis
STEMCELL Technologies RosetteSep™ HLA T Cell Enrichment Cocktail, For processing: 1000 mL whole blood processed
Immunodensity isolation of untouched T cells for HLA analysis